Ts mutations in the genome of cold-adapted variants of human influenza virus A/Krasnodar/101/59 (H2N2) at different passage levels and their reproduction in lungs of hamsters.
Identifieur interne : 002484 ( Main/Exploration ); précédent : 002483; suivant : 002485Ts mutations in the genome of cold-adapted variants of human influenza virus A/Krasnodar/101/59 (H2N2) at different passage levels and their reproduction in lungs of hamsters.
Auteurs : B. Panzig ; L. Döhner ; K B Lisovskaya ; Y Z GhendonSource :
- Acta virologica [ 0001-723X ] ; 1984.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Virus de la grippe A.
- génétique : Virus de la grippe A.
- microbiologie : Poumon.
- Animaux, Basse température, Cellules cultivées, Cricetinae, Gènes viraux, Mutation, Recombinaison génétique, Réplication virale, Sous-type H2N2 du virus de la grippe A, Température.
English descriptors
- KwdEn :
- MESH :
- genetics : Influenza A virus.
- growth & development : Influenza A virus.
- microbiology : Lung.
- Animals, Cells, Cultured, Cold Temperature, Cricetinae, Genes, Viral, Influenza A Virus, H2N2 Subtype, Mutation, Recombination, Genetic, Temperature, Virus Replication.
Abstract
Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperature (33 degrees C). Its reproduction in the lungs of hamsters was decreased as evidenced by approximately 2.5 log10 lower titres; only one of 9 virus isolates from the lungs of hamsters acquired the ts +/- phenotype, although it had retained a ca phenotype. Recombination of this variant with ts mutants of fowl plague virus (FPV) revealed a ts mutation only in gene 4 of this variant coding for haemagglutinin (HA). The virus which had had 25 passages at 28 degrees C possessed the same properties as the previous variant, but all eight virus isolates from the lungs of hamsters retained the ts phenotype; the genome of this variant contained ts mutations in genes 1, 3, 4, 5 and 6. The mutation found in gene 8 was not a ts mutation. The virus, which underwent 25 passages at 28 degrees C and additional 15 passages at 27 degrees C, formed large plaques and alike to the previous variants it possessed the ca and ts phenotypes; however, its reproduction in the lungs of hamsters was decreased by 4.0 log10 and occurred in the lungs only of 4 out 16 infected animals. This variant contained ts mutations in genes 1, 3, 4, 5, 6 and 7 and a non-ts mutation in gene 8.
PubMed: 6151350
Affiliations:
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Le document en format XML
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Döhner</name></author><author><name sortKey="Lisovskaya, K B" sort="Lisovskaya, K B" uniqKey="Lisovskaya K" first="K B" last="Lisovskaya">K B Lisovskaya</name></author><author><name sortKey="Ghendon, Y Z" sort="Ghendon, Y Z" uniqKey="Ghendon Y" first="Y Z" last="Ghendon">Y Z Ghendon</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1984">1984</date><idno type="RBID">pubmed:6151350</idno><idno type="pmid">6151350</idno><idno type="wicri:Area/PubMed/Corpus">000518</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000518</idno><idno type="wicri:Area/PubMed/Curation">000518</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000518</idno><idno type="wicri:Area/PubMed/Checkpoint">000455</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000455</idno><idno type="wicri:Area/Ncbi/Merge">001164</idno><idno type="wicri:Area/Ncbi/Curation">001164</idno><idno type="wicri:Area/Ncbi/Checkpoint">001164</idno><idno type="wicri:doubleKey">0001-723X:1984:Panzig B:ts:mutations:in</idno><idno type="wicri:Area/Main/Merge">002607</idno><idno type="wicri:Area/Main/Curation">002484</idno><idno type="wicri:Area/Main/Exploration">002484</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ts mutations in the genome of cold-adapted variants of human influenza virus A/Krasnodar/101/59 (H2N2) at different passage levels and their reproduction in lungs of hamsters.</title><author><name sortKey="Panzig, B" sort="Panzig, B" uniqKey="Panzig B" first="B" last="Panzig">B. Panzig</name></author><author><name sortKey="Dohner, L" sort="Dohner, L" uniqKey="Dohner L" first="L" last="Döhner">L. Döhner</name></author><author><name sortKey="Lisovskaya, K B" sort="Lisovskaya, K B" uniqKey="Lisovskaya K" first="K B" last="Lisovskaya">K B Lisovskaya</name></author><author><name sortKey="Ghendon, Y Z" sort="Ghendon, Y Z" uniqKey="Ghendon Y" first="Y Z" last="Ghendon">Y Z Ghendon</name></author></analytic><series><title level="j">Acta virologica</title><idno type="ISSN">0001-723X</idno><imprint><date when="1984" type="published">1984</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Cold Temperature</term><term>Cricetinae</term><term>Genes, Viral</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (growth & development)</term><term>Lung (microbiology)</term><term>Mutation</term><term>Recombination, Genetic</term><term>Temperature</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Cellules cultivées</term><term>Cricetinae</term><term>Gènes viraux</term><term>Mutation</term><term>Poumon (microbiologie)</term><term>Recombinaison génétique</term><term>Réplication virale</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Température</term><term>Virus de la grippe A (croissance et développement)</term><term>Virus de la grippe A (génétique)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Cold Temperature</term><term>Cricetinae</term><term>Genes, Viral</term><term>Influenza A Virus, H2N2 Subtype</term><term>Mutation</term><term>Recombination, Genetic</term><term>Temperature</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Cellules cultivées</term><term>Cricetinae</term><term>Gènes viraux</term><term>Mutation</term><term>Recombinaison génétique</term><term>Réplication virale</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human influenza virus A/Krasnodar/101/59 (H2N2) was passaged in chick fibroblast cultures in the presence of trypsin at suboptimal temperature. The virus which underwent 16 passages at 28 degrees C possessed cold-adapted (ca) and temperature sensitive (ts) phenotypes and formed larger plaques at the optimal temperature (33 degrees C). Its reproduction in the lungs of hamsters was decreased as evidenced by approximately 2.5 log10 lower titres; only one of 9 virus isolates from the lungs of hamsters acquired the ts +/- phenotype, although it had retained a ca phenotype. Recombination of this variant with ts mutants of fowl plague virus (FPV) revealed a ts mutation only in gene 4 of this variant coding for haemagglutinin (HA). The virus which had had 25 passages at 28 degrees C possessed the same properties as the previous variant, but all eight virus isolates from the lungs of hamsters retained the ts phenotype; the genome of this variant contained ts mutations in genes 1, 3, 4, 5 and 6. The mutation found in gene 8 was not a ts mutation. The virus, which underwent 25 passages at 28 degrees C and additional 15 passages at 27 degrees C, formed large plaques and alike to the previous variants it possessed the ca and ts phenotypes; however, its reproduction in the lungs of hamsters was decreased by 4.0 log10 and occurred in the lungs only of 4 out 16 infected animals. This variant contained ts mutations in genes 1, 3, 4, 5, 6 and 7 and a non-ts mutation in gene 8.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Dohner, L" sort="Dohner, L" uniqKey="Dohner L" first="L" last="Döhner">L. Döhner</name><name sortKey="Ghendon, Y Z" sort="Ghendon, Y Z" uniqKey="Ghendon Y" first="Y Z" last="Ghendon">Y Z Ghendon</name><name sortKey="Lisovskaya, K B" sort="Lisovskaya, K B" uniqKey="Lisovskaya K" first="K B" last="Lisovskaya">K B Lisovskaya</name><name sortKey="Panzig, B" sort="Panzig, B" uniqKey="Panzig B" first="B" last="Panzig">B. Panzig</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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